Molecular sexing of the diversified avian family Strigidae is difficult. Sex identification using the intron length difference between W and Z chromosomal CHD1 genes, as visualized by agarose gel electrophoreses, often produces ambiguous results. Here we describe a simple method for sexing a variety of Strigidae species using oligonucleotide microarrays, on which several sex-specific probes operated complementarily or in concert. The sex of 8 owl species was identified clearly on the microarrays through sequence recognition. This sequence-directed method can be easily applied to a wider range of Strigidae species. DNA microarrays are miniaturized microsystems based on the ability of DNA to spontaneously find and bind to its complementary sequence by hybridization.
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For Owls, as with other birds, the period during which nesting and rearing young occurs is the most important part of the annual cycle. It is the time during which genes are passed on to the next generation, and considerable effort is expended by the parents to make sure this happens. In the case of most Owl species, especially those found in temperate or sub-Arctic regions, breeding occurs during the spring. However, all the upbringing of their young, and the period immediately following their fledging, is invariably timed to coincide with the maximum abundance of prey animals.
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